Journal: Journal of nanobiotechnology

Article Title: Carbon dots from purple sweet potato as a promising anti-inflammatory biomaterial for alleviating the LPS-induced inflammation in macrophages.

doi: 10.1186/s12951-025-03494-9

Figure Lengend Snippet: Fig. 3 Effects of CPP-CDs on LPS-induced inflammation. a. ELISA measurements of IL-1β, IL-6, and TNF-α protein levels in BMDMs culture supernatants. Experimental groups were treated with LPS (1 µg/mL, 4 h), Nigericin (Nig, 5µM, 45 min), and various concentrations of CPP-CDs (n = 4 biological repli cates). b-c. ATP levels were measured using an ATP assay kit to assess intracellular and extracellular ATP levels in BMDMs (n = 4 biological replicates). d. ROS levels in BMDMs were measured using a ROS detection probe (n = 3 biological replicates). e. Morphological changes in BMDMs were observed under an optical microscope for each treatment group. Red arrows indicate pyroptotic cells (n = 3 biological replicates). Scale bar: 20 μm. f. LDH release levels in BMDMs for each treatment group, represented as the percentage of LDH activity in the cell lysate (n = 4 biological replicates). g. The relative expres sion of M2 macrophage markers ARG1 and CD206 was measured by RT-qPCR in BMDMs treated with various concentrations of CPP-CDs (n = 3 biological replicates). a-g. Data are expressed as mean ± standard error of the mean (SEM). One-way or Two-way ANOVA was used for multiple group comparisons. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: The levels of IL-1β, IL-6 and TNF-α in cell culture supernatants and mouse serum were measured using ELISA kits (Proteintech).

Techniques: Enzyme-linked Immunosorbent Assay, ATP Assay, Microscopy, Activity Assay, Quantitative RT-PCR

Journal: Journal of nanobiotechnology

Article Title: Carbon dots from purple sweet potato as a promising anti-inflammatory biomaterial for alleviating the LPS-induced inflammation in macrophages.

doi: 10.1186/s12951-025-03494-9

Figure Lengend Snippet: Fig. 4 In vivo anti-inflammatory effects of CPP-CDs in an LPS-induced mouse model. a. Mouse model construction process: Mice were divided into groups and given intraperitoneal injections of low (L: 15 mg/kg) or high (H: 30 mg/kg) concentrations of CPP-CDs, followed by LPS injection (5 mg/kg) 2 h later. b. Survival rate of mice monitored for 72 h after LPS and CPP-CDs treatment (n = 6, two independent experiments). c. Colon and liver tissues were collected 24 h after LPS and CPP-CDs treatment and stained with H&E to observe tissue damage (magnification: 15×). d. Pathological scoring of colonic lesions and liver inflammation. Colon score was based on weight loss, rectal bleeding, and stool consistency; liver score was based on the number and area of inflammatory foci around the central vein (n = 6, two independent experiments). e. ELISA measurements of IL-1β, IL-6, and TNF-α levels in mouse serum (n = 4 biological replicates). b, d-e. All data are expressed as mean ± standard error of the mean (SEM). One-way or Two-way ANOVA was used for multiple group comparisons. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: The levels of IL-1β, IL-6 and TNF-α in cell culture supernatants and mouse serum were measured using ELISA kits (Proteintech).

Techniques: In Vivo, Injection, Staining, Enzyme-linked Immunosorbent Assay

Journal: Journal of cellular and molecular medicine

Article Title: The Impact of METTL3 on MDM2 Promotes Podocytes Injury During Diabetic Kidney Disease.

doi: 10.1111/jcmm.70627

Figure Lengend Snippet: FIGURE 5 | Inhibiting podocytes MDM2 expression alleviates renal pathological damage, and reduces podocytes dedifferentiation levels. (A) Representative images showing PAS and Masson staining (scale bar = 20 μm) in control, STZ, and shMDM2-injected STZ groups. (B) IF staining of Ki-67 (red), Cyclin B1 (green), Podocin (yellow), and MDM2 (pink), counterstained with DAPI (blue) in control, STZ and STZ injected with shMDM2 groups. (C) Protein levels of p21, Cyclin B1 and Podocin in control, STZ and STZ injected with shMDM2 groups, analysed by western blot. Data rep- resent mean ± SD of three independent experiments.

Article Snippet: The following antibodies were used at these dilutions for western blot analysis: METTL3 (Abcam ab195352, 1:1000), METTL14 (Sigma, HPA038002, 1:1000), FTO (Proteintech, 27226- 1- AP, 1:1000), synaptopodin (synap) (Sigma- Aldrich, SAB3500585, 1:500), podocin (Abcam, ab181143, 1:500), caspase 3 (Abcam, ab184787, 1:500), B- cell lymphoma/leukaemia- 2 (Bcl2) (Abcam, ab182858, 1:1000), MDM2 (Santa Cruz Biotechnology, sc- 965, 1:500), IGF2BP2 (Proteintech, 11,601- 1- AP, 1:1000), proliferating cell nuclear antigen (PCNA) (Abcam, ab92552, 1:1000), cyclin B1 (Abcam, ab181593, 1:500), cyclin- dependent kinase inhibitor 1A (P21) (Proteintech, 28,248- 1- AP for mouse, 1:1000), Notch intracellular domain (NICD) (Abcam, ab52627, 1:500), and hairy and enhancer of split 1 (Hes1) (Abcam, ab71559, 1:500).

Techniques: Expressing, Staining, Control, Injection, Western Blot

Journal: Journal of cellular and molecular medicine

Article Title: The Impact of METTL3 on MDM2 Promotes Podocytes Injury During Diabetic Kidney Disease.

doi: 10.1111/jcmm.70627

Figure Lengend Snippet: FIGURE 6 | MDM2 regulates the Notch1 signalling pathway in podocytes during AGE-induced abnormal cell cycle regulation. (A, B) Western blot analysis and semi-quantitative assessment of NICD and Hes1 protein levels in BSA-treated, AGE-treated, and siMDM2-transfected AGE-treated groups. (C, D) Western blot analysis and semi-quantitative assessment of NICD and Hes1 protein levels in control, STZ-treated, and shMDM2-injected STZ-treated groups. (E) Evaluation of NICD and Hes1 protein levels in control, STZ-treated and shMDM2-injected STZ-treated groups by IHC as- say (scale bar = 50 μm). (F, G) Western blot analysis and semi-quantitative assessment of Hes1, Cyclin B1 and p-H3 protein levels in AGE-treated, AGE + siMDM2-treated, and Jagged1-treated AGE + siMDM2 groups. Data represent mean ± SD of three independent experiments. **p < 0.01 versus AGE group (B), or STZ group (D), or AGE + siMDM2 group (G) by one-way ANOVA.

Article Snippet: The following antibodies were used at these dilutions for western blot analysis: METTL3 (Abcam ab195352, 1:1000), METTL14 (Sigma, HPA038002, 1:1000), FTO (Proteintech, 27226- 1- AP, 1:1000), synaptopodin (synap) (Sigma- Aldrich, SAB3500585, 1:500), podocin (Abcam, ab181143, 1:500), caspase 3 (Abcam, ab184787, 1:500), B- cell lymphoma/leukaemia- 2 (Bcl2) (Abcam, ab182858, 1:1000), MDM2 (Santa Cruz Biotechnology, sc- 965, 1:500), IGF2BP2 (Proteintech, 11,601- 1- AP, 1:1000), proliferating cell nuclear antigen (PCNA) (Abcam, ab92552, 1:1000), cyclin B1 (Abcam, ab181593, 1:500), cyclin- dependent kinase inhibitor 1A (P21) (Proteintech, 28,248- 1- AP for mouse, 1:1000), Notch intracellular domain (NICD) (Abcam, ab52627, 1:500), and hairy and enhancer of split 1 (Hes1) (Abcam, ab71559, 1:500).

Techniques: Western Blot, Transfection, Control, Injection